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    Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10259/4994

    Título
    Insulin degrading enzyme is up-regulated in pancreatic β cells by insulin treatment
    Autor
    Fernández Díaz, Cristina M. .
    Escobar Curbelo, Luis .
    López Acosta, J.F.
    Lobatón, Carmen D.
    Moreno, Alfredo
    Sanz Ortega, Julián
    Perdomo Hernández, Germán M.Autoridad UBU Orcid
    Cózar Castellano, Irene
    Publicado en
    Histology and Histopathology. 2018, V. 33, n. 11, p. 1167-1180
    Editorial
    Universidad de Murcia
    Fecha de publicación
    2018-11
    ISSN
    0213-3911
    DOI
    10.14670/HH-11-997
    Zusammenfassung
    Insulin Degrading Enzyme (IDE) is an endopeptidase that degrades insulin and glucagon. Ide gene has been associated with type-2 diabetes mellitus (DM2). However, the physiological role(s) of IDE in glucose homeostasis and its potential therapeutic benefit remain not completely known. To contribute in the understanding of IDE's role in glucose metabolism, we analyzed IDE protein level in pancreatic islets from two hyperinsulinemic mouse models, db/db and high-fat diet (HFD) mice, as well as in human islets from DM2 patients treated with oral hypoglycemic agents (OHAs) or insulin. IDE protein level was detected by staining and by western-blot. INS1E cells, rat and human islets were treated with insulin and IDE protein level was studied. We have shown for the first time IDE staining in rodent and human tissue, using the proper negative control, IDE null mouse tissue. Our staining indicates that IDE is expressed in both beta- and alpha-cells, with higher expression in alpha-cells. Db/db and HFD mice islets showed increased IDE protein level. Interestingly, human islets from DM2 patients treated with OHAs showed decreased IDE protein level in beta-cells. Meanwhile, islets from insulin-treated DM2 patients showed augmented IDE protein level compared to OHAs patients, pointing to an upregulation of IDE protein level stimulated by insulin. These data correlate nicely with insulin-stimulated upregulation of IDE in cultured INS1E cells, as well as in rat and human islets.In conclusion, our study shows that IDE is expressed in pancreatic beta- and alpha-cells of both rodents and humans, having higher expression in alpha-cells. Furthermore, insulin stimulates IDE protein level in pancreatic beta-cells. These results may have implications in how DM2 patient’s treatment affects their beta-cell function.
    Palabras clave
    Insulin-degrading enzyme
    Type 2 diabetes,
    Insulin treatment,
    OHAs
    Beta-cells
    Alpha-cells
    Rodent islets
    Human islets
    Materia
    Endocrinología
    Endocrinology
    URI
    http://hdl.handle.net/10259/4994
    Versión del editor
    http://www.hh.um.es/2018/HH_33_11_2018.htm
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    Attribution-NonCommercial-NoDerivatives 4.0 International
    Documento(s) sujeto(s) a una licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    Dateien zu dieser Ressource
    Nombre:
    Fernandez-hh_2018.pdf
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    20.33Mb
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