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    Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10259/5216

    Título
    Interaction analysis of commercial graphene oxide nanoparticles with unicellular systems and biomolecules
    Autor
    Domi, BrixhildaAutoridad UBU Orcid
    Rumbo Lorenzo, CarlosAutoridad UBU Orcid
    García Tojal, JavierAutoridad UBU Orcid
    Elena Sima, Livia
    Negroiu, Gabriela
    Tamayo Ramos, Juan AntonioAutoridad UBU Orcid
    Publicado en
    International Journal of Molecular Sciences. 2020, V. 21, n. 1, 205
    Editorial
    MDPI
    Fecha de publicación
    2020-01
    DOI
    10.3390/ijms21010205
    Résumé
    The ability of commercial monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) to interact with different unicellular systems and biomolecules was studied by analyzing the response of human alveolar carcinoma epithelial cells, the yeast Saccharomyces cerevisiae and the bacteria Vibrio fischeri to the presence of different nanoparticle concentrations, and by studying the binding affinity of different microbial enzymes, like the α-l-rhamnosidase enzyme RhaB1 from the bacteria Lactobacillus plantarum and the AbG β-d-glucosidase from Agrobacterium sp. (strain ATCC 21400). An analysis of cytotoxicity on human epithelial cell line A549, S. cerevisiae (colony forming units, ROS induction, genotoxicity) and V. fischeri (luminescence inhibition) cells determined the potential of both nanoparticle types to damage the selected unicellular systems. Also, the protein binding affinity of the graphene derivatives at different oxidation levels was analyzed. The reported results highlight the variability that can exist in terms of toxicological potential and binding affinity depending on the target organism or protein and the selected nanomaterial.
    Palabras clave
    Graphene
    Unicellular organisms
    Toxicity
    Binding capacity
    ATR-FTIR
    TEM
    ICP-MS
    Materia
    Materia-Composición
    Matter-Constitution
    URI
    http://hdl.handle.net/10259/5216
    Versión del editor
    https://doi.org/10.3390/ijms21010205
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    Domi-ijms_2020.pdf
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