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dc.contributor.authorRumbo Lorenzo, Carlos 
dc.contributor.authorTamayo Ramos, Juan Antonio 
dc.contributor.authorCaso, Federica
dc.contributor.authorRinaldi, Antonio
dc.contributor.authorRomero Santacreu, Lorena 
dc.contributor.authorQuesada Pato, Roberto 
dc.contributor.authorCuesta López, Santiago 
dc.date.accessioned2018-07-25T10:25:06Z
dc.date.available2019-04-01T02:45:07Z
dc.date.issued2018-04
dc.identifier.issn1944-8244
dc.identifier.urihttp://hdl.handle.net/10259/4866
dc.description.abstractElectrospun biodegradable polymers have emerged as promising materials for their applications in several fields, including biomedicine and food industry. For this reason, the susceptibility of these materials to be colonized by different pathogens is a critical issue for public health, and their study can provide future knowledge to develop new strategies against bacterial infections. In this work, the ability of three pathogenic bacterial species (Pseudomonas aeruginosa, Acinetobacter baumannii, and Listeria monocytogenes) to adhere and form biofilm in electrospun polycaprolactone (PCL) microfibrous meshes was investigated. Bacterial attachment was analyzed in meshes with different microstructure, and comparisons with other materials (borosilicate glass and electrospun polylactic acid (PLA)) fibers were assessed. Analysis included colony forming unit (CFU) counts, scanning electron microscopy (SEM), and crystal violet (CV) staining. All the obtained data suggest that PCL meshes, regardless of their microstructure, are highly susceptible to be colonized by the pathogenic relevant bacteria used in this study, so a pretreatment or a functionalization with compounds that present some antimicrobial activity or antibiofilm properties is highly recommended before their application. Moreover, an experiment designed to simulate a chronic wound environment was used to demonstrate the ability of these meshes to detach biofilms from the substratum where they have developed, thus making them promising candidates to be used in wound cleaning and disinfection.en
dc.description.sponsorshipEuropean Union’s H2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 691095 and Junta de Castilla y Leon-FEDER (projects BU079U16 and BU092U16).en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherAmerican Chemical Societyen
dc.relation.ispartofACS Applied Materials & Interfaces. 2018, V. 10, n. 14, p. 11467–11473en
dc.subjectbacterial attachmenten
dc.subjectbiofilmen
dc.subjectelectrospun polycaprolactoneen
dc.subjectfoodborne pathogensen
dc.subjectmicrofibersen
dc.subjectnosocomial pathogensen
dc.subject.otherQuímica orgánicaes
dc.subject.otherChemistry, Organicen
dc.subject.otherMaterialeses
dc.subject.otherMaterialsen
dc.titleColonization of electrospun polycaprolactone fibers by relevant pathogenic bacterial strainsen
dc.typeArtículoes
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1021/acsami.7b19440
dc.identifier.doi10.1021/acsami.7b19440
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/691095
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU079U16
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU092U16
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersionen


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