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dc.contributor.authorAntón Millán, Noemí 
dc.contributor.authorGarcía Tojal, Javier 
dc.contributor.authorMarty Roda, Marta 
dc.contributor.authorGarroni, Sebastiano 
dc.contributor.authorCuesta López, Santiago 
dc.contributor.authorTamayo Ramos, Juan Antonio 
dc.date.accessioned2018-07-25T11:12:12Z
dc.date.available2019-06-01T02:45:06Z
dc.date.issued2018-05
dc.identifier.issn1944-8244
dc.identifier.urihttp://hdl.handle.net/10259/4867
dc.description.abstractThe modification of carbon nanomaterials with biological molecules paves the way toward their use in biomedical and biotechnological applications, such as next-generation biocatalytic processes, development of biosensors, implantable electronic devices, or drug delivery. In this study, different commercial graphene derivatives, namely, monolayer graphene oxide (GO), graphene oxide nanocolloids (GOCs), and polycarboxylate-functionalized graphene nanoplatelets (GNs), were compared as biomolecule carrier matrices. Detailed spectroscopic analyses showed that GO and GOC were similar in composition and functional group content and very different from GN, whereas divergent morphological characteristics were observed for each nanomaterial through microscopy analyses. The commercial α-l-rhamnosidase RhaB1 from the probiotic bacterium Lactobacillus plantarum, selected as a model biomolecule for its relevant role in the pharma and food industries, was directly immobilized on the different materials. The binding efficiency and biochemical properties of RhaB1–GO, RhaB1–GOC, and RhaB1–GN composites were analyzed. RhaB1–GO and RhaB1–GOC showed high binding efficiency, whereas the enzyme loading on GN, not tested in previous enzyme immobilization studies, was low. The enzyme showed contrasting changes when immobilized on the different material supports. The effect of pH on the activity of the three RhaB1-immobilized versions was similar to that observed for the free enzyme, whereas the activity–temperature profiles and the response to the presence of inhibitors varied significantly between the RhaB1 versions. In addition, the apparent Km for the immobilized and soluble enzymes did not change. Finally, the free RhaB1 and the immobilized enzyme in GOC showed the best storage and reutilization stability, keeping most of their initial activity after 8 weeks of storage at 4 °C and 10 reutilization cycles, respectively. This study shows, for the first time, that distinct commercial graphene derivatives can influence differently the catalytic properties of an enzyme during its immobilization.en
dc.description.sponsorshipEuropean Union’s H2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 691095, Ministerio de Economiá y Competitividad (CTQ2016-75023-C2-1-P, CTQ2015-70371- REDT MetDrugs Network), and Junta de Castilla y Leon- FEDER grants BU076U16, BU079U16 and UBU-11-A.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherAmerican Chemical Societyen
dc.relation.ispartofACS Applied Materials & Interfaces. 2018, V. 10, n. 21, p. 18170–18182en
dc.subjectATR-FTIRen
dc.subjectbiocatalysisen
dc.subjectgrapheneen
dc.subjectimmobilizationen
dc.subjectTEMen
dc.subjectα-l-rhamnosidaseen
dc.subject.otherQuímica inorgánicaes
dc.subject.otherChemistry, Inorganicen
dc.subject.otherMaterialeses
dc.subject.otherMaterialsen
dc.titleInfluence of three commercial graphene derivatives on the catalytic properties of a lactobacillus plantarum α-l-Rhamnosidase when used as immobilization matricesen
dc.typeArtículoes
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1021/acsami.7b18844
dc.identifier.doi10.1021/acsami.7b18844
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/691095
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CTQ2016-75023-C2-1-P
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CTQ2015-70371- REDT
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU079U16
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU076U16
dc.relation.projectIDUBU-11-A
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersionen


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