Excitation-emission matrix (EEM) fluorescence spectroscopy has been applied to characterize several urban and industrial wastewaters (effluents from different types of industries: brewery, winery, dairy, biscuit, tinned fish industry, slaughterhouse, pulp mill, textile dyeing and landfill leachates), searching for specific fluorescence fingerprints. Tryptophan protein-like peaks (T1 and T2) are the predominant fluorescence in urban and food industry wastewaters (brewery, winery, dairy/milk, biscuit and fish farm industries) but no special fingerprint has been found to discriminate among them. Protein-like fluorescence also dominates the spectra of meat/fish industries (effluents from a tinned fish industry and a slaughterhouse), but in this case tyrosine protein-like peaks (B1 and B2) also appear in the spectra in addition to tryptophan-like peaks. This fact might constitute a specific feature to differentiate these wastewaters from others, since the appearance of peaks B is quite uncommon in wastewaters. The textile dyeing effluent shows a characteristic triple humic-like fluorescence (peaks A, C1 and C2) that may represent a specific fingerprint for this kind of effluent. Leachates from medium-aged and old landfills might also show a specific fingerprint in their EEM spectra: the sole presence of the humic-like peak C with very high fluorescence intensity. This feature also allows differentiating them from young landfill leachates, which show predominance of protein-like peaks. The fluorescence index (FI) does not seem to be very appropriate to characterize wastewaters and its usefulness might be limited to the study of humic substances in natural waters, although further studies are needed on this topic. However, the humification index (HIX) and the biological index (BIX) do seem to be useful for studying wastewaters, since they have provided consistent results in the present work. This study shows the potential of EEM fluorescence to identify the origin of some industrial effluents, although more research is needed to check these preliminary results.
