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Título : Barley seed coating with free and immobilized alkaline phosphatase to improve P uptake and plant growth
Autor : Pilar Izquierdo, María Concepción
Ortega Santamaría, Natividad
Pérez Mateos, Manuel
Busto Núñez, Mª Dolores
Publicado en: The Journal of Agricultural Science, v. 150, n. 6 (dic. 2012), pp 691-701
Editorial : Cambridge University Press
Fecha de publicación : feb-2012
ISSN : 0021-8596
DOI: 10.1017/S0021859612000019
Resumen : Coating barley seeds with free and immobilized alkaline phosphatase was investigated as a potential means to enhance plant utilization of accumulated soil phosphorus (P). Two coating techniques were studied: film-coating and pelleting. The highest phosphatase activity retention in the coating layer, ranging from 0·48 to 0·67, was observed when seeds were film-coated with phosphatase–polyresorcinol complex (PPC). The germination of seeds film-coated or pelleted with alkaline phosphatase ranged from 0·84 to 0·97 or 0·14 to 0·25, respectively. Low germination of the pelleted seeds was attributed to freezing the seeds in liquid nitrogen (N) for the layer coating formation. Pelleted seeds were not used in the remainder of the studies. Under pot culture conditions, an increase in the soil inorganic P was detected when the seeds were film-coated with phosphatase. Moreover, the film-coating significantly increased the P uptake by plants (between 25 and 31% after 35 days after planting (DAP)). The present study showed that the seed film-coating with free and immobilized phosphatase increased the phosphatase activity in the rhizosphere and the P uptake by plants.
Materia: Bioquímica
Biochemistry
Derechos: © Cambridge University Press, 2012
URI : http://hdl.handle.net/10259.4/2518
Versión del editor: http://dx.doi.org/10.1017/S0021859612000019
Aparece en las colecciones: Artículos BBT

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