Universidad de Burgos RIUBU Principal Default Universidad de Burgos RIUBU Principal Default
  • español
  • English
  • français
  • Deutsch
  • português (Brasil)
  • italiano
Universidad de Burgos RIUBU Principal Default
  • Ayuda
  • Contactez-nous
  • Faire parvenir un commentaire
  • Acceso abierto
    • Archivar en RIUBU
    • Acuerdos editoriales para la publicación en acceso abierto
    • Controla tus derechos, facilita el acceso abierto
    • Sobre el acceso abierto y la UBU
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Parcourir

    Tout RIUBUCommunautés & CollectionsPar date de publicationAuteursTitresSujetsCette collectionPar date de publicationAuteursTitresSujets

    Mon compte

    Ouvrir une sessionS'inscrire

    Statistiques

    Statistiques d'usage de visualisation

    Compartir

    Voir le document 
    •   Accueil de RIUBU
    • E-Prints
    • Untitled
    • Untitled
    • Artículos BIOIND
    • Voir le document
    •   Accueil de RIUBU
    • E-Prints
    • Untitled
    • Untitled
    • Artículos BIOIND
    • Voir le document

    Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10259/5045

    Título
    Pectin methylesterase inactivation by High Pressure Carbon Dioxide (HPCD)
    Autor
    Benito Román, OscarAutoridad UBU Orcid
    Sanz Díez, Mª TeresaAutoridad UBU Orcid
    Illera Gigante, Alba EsterAutoridad UBU Orcid
    Melgosa Gómez, RodrigoAutoridad UBU Orcid
    Benito Moreno, José ManuelAutoridad UBU Orcid
    Beltrán Calvo, SagrarioAutoridad UBU Orcid
    Publicado en
    The Journal of Supercritical Fluids. 2019, V. 145, p. 111-121
    Editorial
    Elsevier
    Fecha de publicación
    2019-03
    ISSN
    0896-8446
    DOI
    10.1016/j.supflu.2018.11.009
    Résumé
    In the present work the effect of High Pressure Carbon Dioxide (HPCD) on the activity and structure of a commercial pectin methylesterase (PME) was studied and the results were compared to the conventional thermal inactivation technology. The use of supercritical CO2 (pressure 6–18 MPa, temperature 40–55 °C and time up to 75 min) increased dramatically the PME inactivation rate. The experimental data fitted the first order model and the inactivation kinetic study of PME was completed with the calculations of the activation energy and volume of activation. HPCD treatment induced important changes in the tertiary structure of the enzyme, as revealed the intrinsic fluorescence, KI quenching and ANS binding analyses. They showed that HPCD treatment rearranged the structure of the enzyme. These changes induced by HPCD were significantly different from those induced by mild thermal treatment. Finally, the structural changes detected correlated with the enzyme activity losses observed.
    Palabras clave
    PME
    HPCD
    Enzyme inactivation
    CO2
    Juice
    Materia
    Ingeniería química
    Chemical engineering
    URI
    http://hdl.handle.net/10259/5045
    Versión del editor
    https://doi.org/10.1016/j.supflu.2018.11.009
    Aparece en las colecciones
    • Artículos BIOIND
    Attribution-NonCommercial-NoDerivatives 4.0 International
    Documento(s) sujeto(s) a una licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    Fichier(s) constituant ce document
    Nombre:
    Benito-tjsf_2019.pdf
    Tamaño:
    725.7Ko
    Formato:
    Adobe PDF
    Thumbnail
    Voir/Ouvrir

    Métricas

    Citas

    Academic Search
    Ver estadísticas de uso

    Exportar

    RISMendeleyRefworksZotero
    • edm
    • marc
    • xoai
    • qdc
    • ore
    • ese
    • dim
    • uketd_dc
    • oai_dc
    • etdms
    • rdf
    • mods
    • mets
    • didl
    • premis
    Afficher la notice complète