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dc.contributor.authorHerrero Gutiérrez, Ana 
dc.contributor.authorSanllorente Méndez, Silvia 
dc.contributor.authorReguera Alonso, Celia 
dc.contributor.authorOrtiz Fernández, Mª Cruz 
dc.contributor.authorSarabia Peinador, Luis Antonio 
dc.date.accessioned2017-03-14T08:27:33Z
dc.date.available2018-11-01T03:45:05Z
dc.date.issued2016-11
dc.identifier.urihttp://hdl.handle.net/10259/4357
dc.description.abstractA new strategy to approach multiresponse optimization in conjunction to a D-optimal design for simultaneously optimizing a large number of experimental factors is proposed. The procedure is applied to the determination of biogenic amines (histamine, putrescine, cadaverine, tyramine, tryptamine, 2- phenylethylamine, spermine and spermidine) in swordfish by HPLC-FLD after extraction with an acid and subsequent derivatization with dansyl chloride. Firstly, the extraction from a solid matrix and the derivatization of the extract are optimized. Ten experimental factors involved in both stages are studied, seven of them at two levels and the remaining at three levels; the use of a D-optimal design leads to optimize the ten experimental variables, significantly reducing by a factor of 67 the experimental effort needed but guaranteeing the quality of the estimates. A model with 19 coefficients, which includes those corresponding to the main effects and two possible interactions, is fitted to the peak area of each amine. Then, the validated models are used to predict the response (peak area) of the 3456 experiments of the complete factorial design. The variability among peak areas ranges from 13.5 for 2-phenylethylamine to 122.5 for spermine, which shows, to a certain extent, the high and different effect of the pretreatment on the responses. Then the percentiles are calculated from the peak areas of each amine. As the experimental conditions are in conflict, the optimal solution for the multiresponse optimization is chosen from among those which have all the responses greater than a certain percentile for all the amines. The developed procedure reaches decision limits down to 2.5 mg L 1 for cadaverine or 497 mg L 1 for histamine in solvent and 0.07 mg kg 1 and 14.81 mg kg 1 in fish (probability of false positive equal to 0.05), respectively.en
dc.description.sponsorshipMINECO (CTQ2014-53157-R) and FEDER fundsen
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieren
dc.relation.ispartofAnalytica Chimica Acta. 2016, V. 945, p. 31–38en
dc.subjectMultiresponse optimizationen
dc.subjectD-optimal designen
dc.subjectBiogenic aminesen
dc.subjectHPLC-FLDen
dc.subjectDerivatizationen
dc.subjectFishen
dc.titleA new multiresponse optimization approach in combination with a D-Optimal experimental design for the determination of biogenic amines in fish by HPLC-FLDen
dc.typeArtículoes
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.aca.2016.10.001
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersionen


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