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dc.contributor.author | Barquero Quirós, Miriam | |
dc.contributor.author | Domínguez Renedo, Olga | |
dc.contributor.author | Alonso Lomillo, Mª Asunción | |
dc.contributor.author | Arcos Martínez, Julia | |
dc.date.accessioned | 2017-03-20T08:50:47Z | |
dc.date.available | 2017-03-20T08:50:47Z | |
dc.date.issued | 2014-05 | |
dc.identifier.uri | http://hdl.handle.net/10259/4380 | |
dc.description.abstract | A novel amperometric biosensor for the determination of Al(III) based on the inhibition of the enzyme acetylcholinesterase has been developed. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles. The oxidation signal of acetylthiocholine iodide enzyme substrate was affected by the presence of Al(III) ions leading to a decrease in the amperometric current. The developed system has a detection limit of 2.1 ± 0.1 μM for Al(III). The reproducibility of the method is 8.1% (n = 4). Main interferences include Mo(VI), W(VI) and Hg(II) ions. The developed method was successfully applied to the determination of Al(III) in spiked tap water . The analysis of a certified standard reference material was also carried out. Both results agree with the certified values considering the respective associated uncertainties. | en |
dc.description.sponsorship | Research Vicerrectory of Costa Rica University (Project 804-B0-197), Junta de Castilla y León (BU212A12-2), Ministerio de Ciencia e Innovación (MICINN) and Fondo Europeo de Desarrollo Regional (FEDER) (INNPACTO SERIBIO 2011–2014) and TEC-2009/12029 | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | es |
dc.publisher | MDPI | en |
dc.relation.ispartof | Sensors, 2014, V. 14, n. 5, p. 8203-8216 | en |
dc.rights | Attribution 3.0 Unported | |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | |
dc.subject | acetylcholinesterase | en |
dc.subject | biosensor | en |
dc.subject | aluminum | en |
dc.subject | acetylthiocholine iodide | en |
dc.subject | screen-printed electrodes | en |
dc.subject | gold nanoparticles | en |
dc.subject.other | Chemistry, Analytic | en |
dc.subject.other | Química analítica | es |
dc.title | Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media | en |
dc.title | Artículo | es |
dc.type | info:eu-repo/semantics/article | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
dc.relation.publisherversion | http://dx.doi.org/10.3390/s140508203 | |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | en |