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dc.contributor.authorQuijada, Narciso M.
dc.contributor.authorFongaro, Gislaine
dc.contributor.authorBarardi, Célia R. M.
dc.contributor.authorHernández Pérez, Marta 
dc.contributor.authorRodríguez Lázaro, David 
dc.date.accessioned2017-04-06T07:23:11Z
dc.date.available2017-04-06T07:23:11Z
dc.date.issued2016-12
dc.identifier.urihttp://hdl.handle.net/10259/4406
dc.description.abstractThe increase of foodborne viral outbreaks highlights the need for a rapid and sensitive method for the prediction of viral infectivity in food samples. This study assesses the use of propidium monoazide (PMA) coupled with real-time PCR methods (RT-qPCR or qPCR for RNA or DNA viruses, respectively) in the determination of viral infectivity in complex animal-related food matrices. Clam and Spanish fermented sausage (“chorizo”) samples were spiked with infectious and heat-inactivated human adenovirus-2 (HAdV-2) and mengovirus (vMC0). PMA-qPCR/RT-qPCR discriminated infective virus particles, with significant reductions (>2.7 log10 or 99.7%). Additionally, infectious HAdV-2 and vMC0 were quantified by plaque assay (in plaque forming units, PFU), and compared with those in virus genomes copies (GCs) quantified by PMA-qPCR/RT-qPCR. A consistent correlation (R2 > 0.92) was showed between PFU and GCs along serial 10-fold dilutions in both DNA and RNA virus and in both food matrices. This study shows the use of PMA coupled to qPCR/RT-qPCR as a promising alternative for prediction of viral infectivity in food samples in comparison to more expensive and time-consuming methods and for those viruses that are not able to grow under available cell culture techniques.en
dc.description.sponsorshipThisstudywasfinanciallysupportedbytheRTA2014-00024- C04-01 fromtheSpanishMinistryofEconomyandInnovation and theBrazilianCNPqProjectnumber472804/2013-8,andby CAPES/PNPD andCAPES/PDSE.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherFrontiers Mediaen
dc.relation.ispartofFrontiers in Microbiology. 2016, V. 7, art. 2008en
dc.subjectenteric virusesen
dc.subjectinfectivity predictionen
dc.subjectPMA-PCRen
dc.subjectclamen
dc.subjectfermented sausageen
dc.titlePropidium Monoazide Integrated with qPCR Enables the Detection and Enumeration of Infectious Enteric RNA and DNA Viruses in Clam and Fermented Sausagesen
dc.typeArtículoes
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2016.02008
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionen


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