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dc.contributor.authorWadsworth, Caroline .
dc.contributor.authorProcopio, Noemi .
dc.contributor.authorAnderung, Cecilia .
dc.contributor.authorCarretero, José Miguel 
dc.contributor.authorIriarte Avilés, Eneko 
dc.contributor.authorValdiosera, Cristina .
dc.contributor.authorRengert Elburg .
dc.contributor.authorKirsty Penkman .
dc.contributor.authorBuckley, Michael
dc.date.accessioned2018-02-02T12:20:48Z
dc.date.available2018-03-01T03:45:06Z
dc.date.issued2017-03
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10259/4724
dc.description.abstractAncient DNA (aDNA) is themost informative biomolecule extracted fromskeletal remains at archaeological sites, but its survival is unpredictable and its extraction and analysis is time consuming, expensive and often fails. Several proposed methods for better understanding aDNA survival are based upon the characterisation of some aspect of protein survival, but these are typically non-specific; proteomic analyses may offer an attractive method for understanding preservation processes. In this study, in-depth proteomic (LC-Orbitrap-MS/MS) analyseswere carried out on 69 archaeological bovine bone and dentine samples from multiple European archaeological sites and comparedwith mitochondrial aDNA and amino acid racemisation (AAR) data. Comparisons of these data, including estimations of the relative abundances for seven selected non-collagenous proteins, indicate that the survival of aDNA in bone or dentine may correlatewith the survival of some proteins, and that proteome complexity is a more useful predictor of aDNA survival than protein abundance or AAR. The lack of a strong correlation between the recovery of aDNA and the proteome abundance may indicate that the survival of aDNA is more closely linked to its ability to associate with bone hydroxyapatite crystals rather than to associate with proteins. Significance: Ancient biomolecule survival remains poorly understood, even with great advancements in ‘omics’ technologies, both in genomics and proteomics. This study investigates the survival of ancient DNA in relation to that of proteins, taking into account proteome complexity and the relative protein abundances to improve our understanding of survival mechanisms. The results show that although protein abundance is not necessarily directly related to aDNA survival, proteome complexity appears to be.en
dc.description.sponsorshipNERC for funding the studentship to CW (NE/J500057/1) and the Royal Society for funding for MB (UF120473 and UF120473). Assistant Professor (CA) funded by the Swedish Research Council Formas (2010-627) and KP by the Leverhulme Trust (PLP-2012-116). The Atapuerca research was supported by the Spanish MINECO (CGL2012-38434-C03-01) project. Fieldwork at the Atapuerca sites was funded by the Junta de Castilla y León and the Fundación Atapuerca.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieren
dc.relation.ispartofJournal of Proteomics.2017, V. 158, p. 1-8en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAncient DNAen
dc.subjectAncient proteinsen
dc.subjectProteomicsen
dc.subjectCollagenen
dc.subjectNon-collagenous proteinsen
dc.titleComparing ancient DNA survival and proteome content in 69 archaeological cattle tooth and bone samples from multiple European sitesen
dc.typeArtículoes
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1016/j.jprot.2017.01.004
dc.identifier.doi10.1016/j.jprot.2017.01.004
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CGL2012-38434-C03-01
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersionen


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