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    Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10259/4787

    Título
    A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification
    Autor
    Pires, Filipa Velez
    Arcos Martínez, JuliaAutoridad UBU Orcid
    Cabral, A. Cristina Dias
    Vidal, Juan C. .
    Castillo, Juan R. .
    Publicado en
    Journal of Pharmaceutical and Biomedical Analysis. 2018, V. 156, p. 372-378
    Editorial
    Elsevier
    Fecha de publicación
    2018-07
    ISSN
    0731-7085
    DOI
    10.1016/j.jpba.2018.04.019
    Resumen
    Human cytomegalovirus (HCMV) is a herpes virus that can cause severe infections. Still, the available methods for its diagnostic have the main disadvantage of requiring long time to be performed. In this work, a simple magnetic particle-based enzyme immunoassay (mpEIA) for the quantification of glycoprotein B of Human cytomegalovirus (gB-HCMV) in urine samples is proposed. The immunosensor scheme is based on the analyte protein gB-HCMV sandwiched between a primary monoclonal antibody, (MBs-PrG-mAb1), and a secondary anti-gB-HCMV antibody labelled with Horseradish peroxidase (Ab2-HRP) to allow spectrophotometric detection. The mpEIA analytical performance was tested in urine samples, showing a linear dependence between gB-HCMV concentration and the absorbance signal at 450 nm in a range of concentrations from 90 to 700 pg mL−1. The calculated detection limits for gB-HCMV were 90 ± 2 pg mL−1 and the RSD was about 6.7% in urine samples. The immunosensor showed good selectivity against other viruses from Herpesviridae family, namely varicella zoster and Epstein Barr viruses. The recoveries of spiked human urine samples at 0.30–0.50 ng mL−1 concentration levels of gB-HCMV ranged between 91 to 105%. The proposed mpEIA method was validated following the guidelines of the European Medicines Agency (EMEA-2014), and allows rapid, successful and easy quantification of gB-HCMV in urine samples.
    Palabras clave
    Magnetic beads
    Immunoassay
    mpEIA
    Human cytomegalovirus
    Glycoprotein B
    Materia
    Chemistry, Analytic
    Química analítica
    URI
    http://hdl.handle.net/10259/4787
    Versión del editor
    https://doi.org/10.1016/j.jpba.2018.04.019
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    • Artículos ELAN
    Attribution-NonCommercial-NoDerivatives 4.0 International
    Documento(s) sujeto(s) a una licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
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    Pires-JPBA_2018.pdf
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