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dc.contributor.authorŁęczkowska, Anna .
dc.contributor.authorGonzález García, Jorge .
dc.contributor.authorPérez Arnáiz, Cristina 
dc.contributor.authorGarcía Ruiz, Begoña 
dc.contributor.authorWhite, Andrew J. P. .
dc.contributor.authorVilar, Ramón .
dc.date.accessioned2018-09-05T09:24:03Z
dc.date.available2019-08-09T02:45:06Z
dc.date.issued2018-08
dc.identifier.issn0947-6539
dc.identifier.urihttp://hdl.handle.net/10259/4911
dc.description.abstractThe proposed in vivo formation of G-quadruplex DNA (G4 DNA) in promoter regions of oncogenes and in telomeres has prompted the development of small molecules with high affinity and selectivity for these structures. Herein we report the synthesis of a new di-substituted bipyridine ligand and the corresponding complexes with Ni2+ and VO2+. Both these new complexes have been characterized spectroscopically and by X-ray crystallography. Detailed DNA binding studies of these two complexes, together with three previously reported metal salphen complexes, are presented. Using FRET melting assays, the binding affinity and selectivity of the five metal complexes against six different G4 DNA structures as well as a duplex DNA have been determined. In addition, we present detailed ITC and UV/Vis studies to characterize the interaction of the complexes with human telomeric G4 DNA. Finally, we show via a polymerase stop assay that these complexes are able to stabilize a G4 DNA structure (from the c-Myc oncogene promoter) and halt the activity of Taq polymerase.en
dc.description.sponsorshipUK’s Engineering and Physical Sciences Research Council (EPSRC) (grant number: EP/H005285/1)en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherWiley-VCH Verlagen
dc.relation.ispartofChemistry-a european journal. 2018, V. 24, n. 45, p. 11785-11794en
dc.subjectcanceren
dc.subjectDNA bindingen
dc.subjectheterocyclesen
dc.subjectmetal complexesen
dc.subjectpolymeraseen
dc.subject.otherQuímica físicaes
dc.subject.otherChemistry, Physical and theoreticalen
dc.titleBinding studies of metal–salphen and metal–bipyridine complexes towards G‐Quadruplex DNAen
dc.typeArtículoes
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1002/chem.201802248
dc.identifier.doi10.1002/chem.201802248
dc.relation.projectIDinfo:eu-repo/grantAgreement/EPSRC/EP/H005285/1
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersionen


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