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dc.contributor.authorMartel Martín, Sonia 
dc.contributor.authorBarros García, Rocío 
dc.contributor.authorDomi, Brixhilda 
dc.contributor.authorRumbo Lorenzo, Carlos 
dc.contributor.authorPoddighe, Matteo
dc.contributor.authorAparicio Martínez, Santiago 
dc.contributor.authorSuarez Diez, Maria
dc.contributor.authorTamayo Ramos, Juan Antonio 
dc.date.accessioned2024-07-25T11:43:51Z
dc.date.available2024-07-25T11:43:51Z
dc.date.issued2021-09-01
dc.identifier.issn2079-4991
dc.identifier.urihttp://hdl.handle.net/10259/9485
dc.description.abstractCarbon nanotubes (CNTs) have attracted the attention of academy and industry due to their potential applications, being currently produced and commercialized at a mass scale, but their possible impact on different biological systems remains unclear. In the present work, an assessment to understand the toxicity of commercial pristine multi-walled carbon nanotubes (MWCNTs) on the unicellular fungal model Saccharomyces cerevisiae is presented. Firstly, the nanomaterial was physico-chemically characterized, to obtain insights concerning its morphological features and elemental composition. Afterwards, a toxicology assessment was carried out, where it could be observed that cell proliferation was negatively affected only in the presence of 800 mg L−1 for 24 h, while oxidative stress was induced at a lower concentration (160 mg L−1) after a short exposure period (2 h). Finally, to identify possible toxicity pathways induced by the selected MWCNTs, the transcriptome of S. cerevisiae exposed to 160 and 800 mg L−1, for two hours, was studied. In contrast to a previous study, reporting massive transcriptional changes when yeast cells were exposed to graphene nanoplatelets in the same exposure conditions, only a small number of genes (130) showed significant transcriptional changes in the presence of MWCNTs, in the higher concentration tested (800 mg L−1), and most of them were found to be downregulated, indicating a limited biological response of the yeast cells exposed to the selected pristine commercial CNTs.en
dc.description.sponsorshipThis work was supported by the European Union’s H2020 research and innovation programme under the grant agreements N° 691095 (NANOGENTOOLS), N° 952379 (SURFBIO), and N° 953152 (DIAGONAL); Junta de Castilla y Leon-FEDER under grant N° BU058P20 (NANOCOMP).en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherMDPIen
dc.relation.ispartofNanomaterials. 2021, V. 11, n. 9, 2272en
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCarbon nanotubesen
dc.subjectMWCNTsen
dc.subjectSaccharomyces cerevisiaeen
dc.subjectToxicityen
dc.subjectOxidative stressen
dc.subjectDifferential expressionen
dc.subjectTranscriptomicsen
dc.subject.otherQuímica físicaes
dc.subject.otherChemistry, Physical and theoreticalen
dc.titleLow Toxicological Impact of Commercial Pristine Multi-Walled Carbon Nanotubes on the Yeast Saccharomyces cerevisiaeen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.3390/nano11092272es
dc.identifier.doi10.3390/nano11092272
dc.identifier.essn2079-4991
dc.journal.titleNanomaterialsen
dc.volume.number11es
dc.issue.number9es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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