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dc.contributor.authorCarrillo Pérez, Celia 
dc.contributor.authorCavia Camarero, María del Mar 
dc.contributor.authorAlonso de la Torre, Sara 
dc.date.accessioned2014-05-29T08:44:59Z
dc.date.available2014-05-29T08:44:59Z
dc.date.issued2013-06
dc.identifier.issn1303–829X
dc.identifier.urihttp://hdl.handle.net/10259.4/2530
dc.description.abstractIntroduction: Econazole, an azole compound widely used as an antifungal drug, is currently investigated for additional therapeutic effects. In fact, the antitumoral properties of econazole have been recently demonstrated, both in vivo and at the cellular level. However, the precise mechanism of action behind its effects is still unclear. Aim: To examine the effect of econazole on intracellular Ca2+ signaling pathways in a human adenocarcinoma cell line. Methods: [Ca2+]i was measured in Fura 2-loaded HT29 cells by fluorescence. Fluorescence was recorded from 1ml aliquots of magnetically stirred HT29 cells using a Cary Eclipse Spectrophotometer (Varian Ltd., Madrid, Spain) with excitation wavelengths of 340 and 380 nm and emission at 510 nm. Results: We have demonstrated that econazole has a dual effect on Ca2+ homeostasis. It caused a dose-dependent increase in [Ca2+]i. Both mobilization from thapsigargin-sensitive stores and extracellular Ca2+ influx contributed to the effect. A phospholipase C-dependent pathway proved to be activated by econazole. In addition, econazole greatly inhibited a physiological activation of store-operated Ca2+ entry. Conclusion: This study provides further molecular mechanism of econazole suggesting that this drug may modify the physiology of human adenocarcinoma HT29 cell line.es
dc.description.sponsorshipJunta de Castilla y Leon (BU001A09), M.E.C. (fellowship C. Carrillo)
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherTurkish Biochemical Societyes
dc.relation.ispartofTürk Biyokimya Dergisi [Turkish Journal of Biochemistry], 2013, v. 38, n. 2, p. 126–132es
dc.subjectEconazolees
dc.subjectCa2+ signalinges
dc.subjectHT29 cellses
dc.subject.otherBiología molecular
dc.subject.otherMolecular biology
dc.titleEffect of econazole on Ca2+ signaling in human colorectal adenocarcinoma cellses
dc.title.alternativeİnsan kolorektal adenokarsinom hücrelerindeki Ca2+ sinyal iletimi üzerine ekonazolün etkisi
dc.typeinfo:eu-repo/semantics/article
dc.rights.holder© Turkish Biochemical Society
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttp://dx.doi.org/10.5505/tjb.2013.08370
dc.identifier.doi10.5505/tjb.2013.08370
dc.relation.projectIDinfo:eu-repo/grantAgreement/JCyL/BU001A09
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionen


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