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dc.contributor.authorMelgosa Gómez, Rodrigo 
dc.contributor.authorSanz Díez, Mª Teresa 
dc.contributor.authorGarcía Solaesa, Ángela 
dc.contributor.authorBucio López, Silvia Liliana 
dc.contributor.authorBeltrán Calvo, Sagrario 
dc.date.accessioned2017-02-13T11:17:22Z
dc.date.available2017-02-13T11:17:22Z
dc.date.issued2015-02
dc.identifier.issn0896-8446
dc.identifier.urihttp://hdl.handle.net/10259/4339
dc.description.abstractThis work investigates the effect of supercritical carbon dioxide (SC-CO2) treatment on four commer-cial lipases. The influence of experimental conditions: temperature (35–70◦C), pressure (10–25 MPa),exposure time (1–3 h) and depressurization steps (1–3) on the residual activity was studied. Activityenhancement was verified for free enzymes (Palatase 20000 L and Lipozyme CALB L) treated at mildconditions; while the highest temperature and pressure and the longest exposure time assayed led toactivity losses. On the other hand, activity losses or no significative effect of the treatment in the enzymeactivity were observed on immobilized enzymes (Lipozyme RM IM and Lipozyme 435). Qualitative stud-ies were also performed: Fluorescence emission spectra showed changes in the conformational structureof both two free enzymes after SC-CO2treatment. Scanning electron micrographs showed morphologicalalterations in the immobilization supports of the treated enzymes; while infrared spectra did not showchemical modifications.en
dc.description.sponsorship(MINECO) and the EuropeanRegional Development Fund (ERDF) for financial support to theproject CTQ2012-39131-C02-01.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieren
dc.relation.ispartofThe Journal of Supercritical Fluids. 2015, V. 97, p. 51–62en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCandida antarctica lipase Ben
dc.subjectRhizomucor miehei lipaseen
dc.subjectImmobilized lipaseen
dc.subjectLipozymeen
dc.subjectEnzyme activityen
dc.subjectSupercritical carbon dioxideen
dc.titleEnzymatic activity and conformational and morphological studies of four commercial lipases treated with supercritical carbon dioxideen
dc.typeArtículoes
dc.typeinfo:eu-repo/semantics/article
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.supflu.2014.11.003
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersionen


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