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    Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10259/4776

    Título
    Potassium‐ion‐selective fluorescent sensors to detect cereulide, the emetic toxin of B. cereus, in food samples and HeLa cells
    Autor
    García Calvo, JoséUBU authority Orcid
    Ibeas Cortes, SaturninoUBU authority Orcid
    Antón García, Eva ClaraUBU authority
    Torroba Pérez, TomásUBU authority Orcid
    González Aguilar, Gerardo .
    Antunes, Wilson .
    González Lavado, Eloísa .
    Fanarraga, Mónica L. .
    Publicado en
    Chemistry Open. 2017, V. 6, n. 4, p. 562-570
    Editorial
    Wiley-VCH Verlag
    Fecha de publicación
    2017-08
    ISSN
    2191-1363
    DOI
    10.1002/open.201700057
    Abstract
    We report the development of new chemical probes for cereulide, a toxic metabolite produced by specific strains of Bacillus cereus, through displacement of potassium cations from a preformed specific complex and a subsequent change in the fluorescence emission. For this purpose, we designed fluorescent probes for potassium cations that were suitable for displacement assays with cereulide from organic extracts. The fluorescence detection of natural cereulide in rice samples was achieved by using synthetic cereulide as a reference and a potassium fluorescent reporter, and this was found to be useful as a portable and fast method for the in situ detection of cereulide in food extracts. To study the fate of cereulide in live cells, we designed a procedure that was suitable for live‐cell microscopy imaging of HeLa cells by comparing the cellular location of the potassium fluorogenic probe, which stained intracellular endolysosomes, in the absence and presence of cereulide; we concluded that in the presence of cereulide, the fluorescence of the probe was decreased because of complexation of the potassium ions by cereulide.
    Materia
    Chemistry
    Química
    URI
    http://hdl.handle.net/10259/4776
    Versión del editor
    https://doi.org/10.1002/open.201700057
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    Attribution-NonCommercial-NoDerivatives 4.0 International
    Documento(s) sujeto(s) a una licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
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