Show simple item record

dc.contributor.authorBenito Román, Oscar 
dc.contributor.authorSanz Díez, Mª Teresa 
dc.contributor.authorMelgosa Gómez, Rodrigo 
dc.contributor.authorPaz Barragán, Esther de 
dc.contributor.authorEscudero Barbero, Isabel 
dc.contributor.authorBeltrán Calvo, Sagrario 
dc.date.accessioned2019-03-20T08:44:13Z
dc.date.issued2019-05
dc.identifier.issn0896-8446
dc.identifier.urihttp://hdl.handle.net/10259/5076
dc.description.abstractTyrosinase from mushroom was used as a model polyphenol oxidase (PPO) enzyme to perform a systematic inactivation study using High Pressure Carbon Dioxide (HPCD). The ratio CO2/volume of enzyme (g/mL) loaded in the reactor was found to be critical. Above a critical ratio, pressure, temperature and time did not control the inactivation performance. Exposure time (2–15 min), temperature (25–45 °C) and pressure (5–20 MPa) allowed to show a characteristic inactivation pattern for PPO: a sudden decrease in activity (at least 75% of the total activity loss was observed within the first 2 min) was followed by a slowed decay. The experimental data were fitted into a two fraction kinetic model and the main kinetic parameters (ZP, ZT activation volume and activation energy) were calculated. The fluorescence spectroscopy analysis of the samples treated with HPCD revealed significant changes in the tertiary structure of the enzyme.en
dc.description.sponsorshipSpanish Government (MINECO) and the European Regional Development Fund (ERDF) for financial support of project CTQ2015-64396-R (MINECO/FEDER, UE). To Junta de Castilla y León and ERDF for O.Benito’s Post-doctoral contract funded by project BU055U16. To MINECO for E. de Paz’s Juan de la Cierva Contract (FJCI-2014-19850).
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieren
dc.relation.ispartofThe Journal of Supercritical Fluids. 2019, V. 147, p. 310-321en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPolyphenol oxidaseen
dc.subjectHPCDen
dc.subjectEnzyme inactivationen
dc.subjectSupercritical carbon dioxideen
dc.subject.otherIngeniería químicaes
dc.subject.otherChemical engineeringen
dc.titleStudies of polyphenol oxidase inactivation by means of high pressure carbon dioxide (HPCD)en
dc.typeArtículoes
dc.typeinfo:eu-repo/semantics/article
dc.date.embargo2021-06
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.relation.publisherversionhttps://doi.org/10.1016/j.supflu.2018.07.026
dc.identifier.doi10.1016/j.supflu.2018.07.026
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CTQ2015-64396-R
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersionen


Files in this item

This item appears in the following Collection(s)

Show simple item record