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dc.contributor.authorPrieto Santiago, Virginia
dc.contributor.authorCavia Camarero, María del Mar 
dc.contributor.authorBarba, Francisco J.
dc.contributor.authorAlonso de la Torre, Sara 
dc.contributor.authorCarrillo Pérez, Celia 
dc.date.accessioned2021-11-22T07:52:22Z
dc.date.available2021-11-22T07:52:22Z
dc.date.issued2022-01
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10259/6196
dc.description.abstractRaffinose family oligosaccharides are non-digestible compounds considered as dietary prebiotics with health-related properties. Hence, it is important to develop highly specific methods for their determination. An analytical method is developed in this study for oligosaccharide identification and quantification using liquid chromatography-tandem mass spectrometry equipped with a triple quadrupole analyser operating in Multiple Reaction Monitoring mode. Raffinose, stachyose and verbascose are separated in a 10-minute run and the method is validated over a broad concentration range, showing good linearity, accuracy, precision and high sensitivity. A low-cost, short eco-friendly procedure for oligosaccharide extraction from legumes, with a high recovery rate extraction, good repeatability and reproducibility is also proposed. No plant-matrix effects were demonstrated. The method applied to the screening of 28 different legumes revealed species-related traits for oligosaccharide distribution, highlighting Pisum sativum (9.22 g/100 g) as the richest source of these prebiotics and its suitability as a functional food ingredient.en
dc.description.sponsorshipPrieto-Santiago is a research assistant funded by Junta de Castilla y León (Spain) (Grant No. UBU-10-B).en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofFood Chemistry. 2022, V. 368, 130761en
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectTriple quadrupoleen
dc.subjectMultiple reaction monitoringen
dc.subjectOligosaccharidesen
dc.subjectLegumesen
dc.subject.otherBiología moleculares
dc.subject.otherMolecular biologyen
dc.subject.otherAlimentoses
dc.subject.otherFooden
dc.titleMultiple reaction monitoring for identification and quantification of oligosaccharides in legumes using a triple quadrupole mass spectrometeren
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1016/j.foodchem.2021.130761es
dc.identifier.doi10.1016/j.foodchem.2021.130761
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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