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    Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10259/4294

    Título
    Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace
    Autor
    Pino García, Raquel delUBU authority
    García Lomillo, JavierUBU authority
    Rivero Pérez, Maria DoloresUBU authority
    González San José, Mª LuisaUBU authority
    Muñiz Rodríguez, PilarUBU authority
    Publicado en
    Journal of Agricultural and Food Chemistry. 2015, V. 63, n. 31, p. 6835-7094
    Editorial
    American Chemical Society
    Fecha de publicación
    2015-08
    ISSN
    0021-8561
    Abstract
    Evaluation of the total antioxidant capacity of solid matrices without extraction steps is a very interesting alternative for food researchers and also for food industries. These methodologies have been denominated QUENCHER from QUick, Easy, New, CHEap, and Reproducible assays. To demonstrate and highlight the validity of QUENCHER (Q) methods, values of Q-method validation were showed for the first time, and they were tested with products of well-known different chemical properties. Furthermore, new QUENCHER assays to measure scavenging capacity against superoxide, hydroxyl, and lipid peroxyl radicals were developed. Calibration models showed good linearity (R2 > 0.995), proportionality and precision (CV < 6.5%), and acceptable detection limits (<20.4 nmol Trolox equiv). The presence of ethanol in the reaction medium gave antioxidant capacity values significantly different from those obtained with water. The dilution of samples with powdered cellulose was discouraged because possible interferences with some of the matrices analyzed may take place.
    Palabras clave
    antioxidant activities
    ABTS
    Folin−Ciocalteu
    free radical scavenging assays
    cellulose
    URI
    http://hdl.handle.net/10259/4294
    Versión del editor
    http://dx.doi.org/10.1021/jf5042678
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