Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10259/7141
Título
Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer
Autor
Publicado en
Food Chemistry. 2023, V. 405, Part A, 134789
Editorial
Elsevier
Fecha de publicación
2023-03
ISSN
0308-8146
DOI
10.1016/j.foodchem.2022.134789
Resumen
Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents’ removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample).
Palabras clave
Catechol
1,2-Dihydroxybenzene
Phenylboronic acid
Glucose oxidase
Catalase
Honey
Materia
Química orgánica
Chemistry, Organic
Química física
Chemistry, Physical and theoretical
Alimentos
Food
Versión del editor
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