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    Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10259/7141

    Título
    Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer
    Autor
    González Ceballos, LaraUBU authority Orcid
    Guirado Moreno, José CarlosUBU authority Orcid
    Utzeri, Gianluca
    García Pérez, José MiguelUBU authority Orcid
    Fernández Muiño, Miguel ÁngelUBU authority Orcid
    Osés Gómez, Sandra MaríaUBU authority Orcid
    Sancho Ortiz, María TeresaUBU authority Orcid
    Arnáiz Alonso, AnaUBU authority Orcid
    Valente, Artur J. M.
    Vallejos Calzada, SaúlUBU authority Orcid
    Publicado en
    Food Chemistry. 2023, V. 405, Part A, 134789
    Editorial
    Elsevier
    Fecha de publicación
    2023-03
    ISSN
    0308-8146
    DOI
    10.1016/j.foodchem.2022.134789
    Abstract
    Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents’ removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample).
    Palabras clave
    Catechol
    1,2-Dihydroxybenzene
    Phenylboronic acid
    Glucose oxidase
    Catalase
    Honey
    Materia
    Química orgánica
    Chemistry, Organic
    Química física
    Chemistry, Physical and theoretical
    Alimentos
    Food
    URI
    http://hdl.handle.net/10259/7141
    Versión del editor
    https://doi.org/10.1016/j.foodchem.2022.134789
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    Atribución 4.0 Internacional
    Documento(s) sujeto(s) a una licencia Creative Commons Atribución 4.0 Internacional
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    Gonzalez-fc_2023.pdf
    Tamaño:
    1.962Mb
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